San Rafael National Park is a 730 km2 reserve that includes the ecorregions of the AtlanticForest and Mesopotamian Grasslands. Over 416 bird species have been recorded in its area, including 27 endangered and near-threatened species. It includes the highestnumber of Atlantic Forest endemic birds in the country.
Cerro Cora National Park holds an interesting mixture of Cerrado and Atlantic Forest habitats, and is also one of the most important sites to get in touch with the history of the country. In its area ocurred the last battle of the Triple Alliance War, where Mariscal Francisco Solano López died.
Mbaracayú Biosphere Reserve includes two of the most important ecorregions in the world: the savannah ecosystems of the Cerrado and the humid Atlantic Forest. These two habitats have high levels of biodiversity as well as very high levels of species endemism. Unfortunately, they are also amongst the most threatened.